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. 2021 Sep 6;12:692424. doi: 10.3389/fphar.2021.692424

FIGURE 8.

FIGURE 8

Inhibition of SIRT1 attenuated the protective effect of 8-MOP on OA rats. The Wistar rat OA model was established by intraarticular injection of 4% papain. Rats were divided into four groups: The OA group, the OA + EX527 (20 μmol/kg body weight) group, the OA + 8-MOP (30 μmol/kg, i.p.) group, the OA + EX527 (20 μmol/kg body weight) +8-MOP (30 μmol/kg, i.p.) group. (A). The PWT was measured to assess the level of bone pain in rats. (B). Caspase3-positive cells were counted by IHC. (C–D). The levels of inflammatory cytokines (IL-6, TNF-α, and IL-18) were detected by qRT-PCR and ELISA. (E–G): The oxidative stress factors (MDA, SOD, and GSH-PX) in the OA site were measured by the MDA, SOD, and GSH-PX detection kits. (H). The expression of SIRT1, p-NF-κB p65 protein levels and acetylation NF-κB p65 was measured by qRT-PCR and Western blot, respectively. *p < 0.05, **p < 0.01, ***p < 0.001. (n = 5).