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. 2021 Sep 6;11:754146. doi: 10.3389/fonc.2021.754146

Figure 6.

Figure 6

E-cadherin is a direct target gene of miR-1227. (A) Upper panel: schematic diagram of the predicted miR-1227 binding site for E-cadherin 3′-UTR. Bottom panel: western blot analysis of E-cadherin expression in pancreatic cancer cells transfected as indicated. (B) Luciferase activity of WT or MUT E-cadherin 3′-UTR in AsPC-1 cells after co-transfection with miR-1227 mimic, and in AsPC-1/Erlo cells after co-transfection with miR-1227 inhibitor. (C) qRT-PCR analysis of E-cadherin expression in AsPC-1/Erlo and AsPC-1 cells. (D) qRT-PCR analysis of E-cadherin expression in pancreatic cancer and normal tissues. (E) qRT-PCR analysis of E-cadherin expression in pancreatic cancer cell lines and normal cells. **P < 0.01, ***P < 0.001.