Figure 2.

Immunohistochemical assay of immune cells in tumor tissues before and immediately after RFA. Before RFA: (a) CD4+ cells, (d) CD8+ cells, (g) CD3+ cells, and (j) NK cells. Immediately after RFA: (b) CD4+ cells, (e) CD8+ cells, (h) CD3+ cells, and (k) NK cells. Quantitative data: (c) CD4+ cells, (f) CD8+ cells, (i) CD3+ cells, and (l) NK cells. Data are presented as mean ± SEM. The brown cells are positive cells. Bar = 100 μm. RFA: radiofrequency ablation, CD: cluster of differentiation, NK: natural killer, SEM: standard error of the mean.