Figure 6:

Measurements of membrane restructuring occurring in DOPC SLBs introduced to 40 μg/mL solutions of rhamnolipid. (A) QCM-D measurements of bilayer reformations initiated by the introduction of rhamnolipid solutions with changes in frequency (ΔFrequency, blue lines; referred to as Δf in the main text for brevity), and dissipation (ΔDissipation, orange lines; referred to as ΔD in the main text for brevity) shown are representative of three or four independent experiments. The initial measurement values (t=0 min) of these experiments correspond to a DOPC SLB on an SiO₂ surface. Rhamnolipid solutions were introduced to the bilayer at the time indicated by the solid arrow and the buffer was introduced at the time indicated by the dashed arrow. (B-E) fluorescence micrographs of bilayer restructuring occurring at (B) 0 sec, (C) 45 sec, and (D) 6 min after the first bilayer restructuring events had been observed or (E) after at least 8 minutes of washing the bilayer with buffer. The direction of flow in all images is from the bottom to the top of the image. Scale bars are 30 μm.