Table 3. Denaturation Temperatures of Type 2 DNA-Dye Constructs.
DNA-dye construct | Tm (°C) |
---|---|
unlabeled (100 mM NaCl added) | 48.5 |
unlabeled (15 mM MgCl2 added) | 60.5 |
monomer | 59.1 |
adjacent dimer | 59.0 |
transverse dimer | 60.9 |
trimer | 60.5 |
tetramer | 62.4 |