Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | NOTCH1 | NCBI | ID: 4851 | |
| Cell line (Homo sapiens) | Human aortic smooth muscle cell line | ATCC | CRL1999, Lot Number: 70019189, RRID:CVCL_4009 |
Female, 11 months old, Caucasian |
| Cell line (Homo sapiens) | Primary human aortic smooth muscle cells | ATCC | PCS100012 | Male, 29 years old, African American |
| Cell line (Homo sapiens) | Primary human aortic smooth muscle cells | This paper | Primary human aortic smooth muscle cells isolated from non-diseased and BAV-TAA patients (Asian). | |
| Transfected construct (human) | NOTCH1 targeted shRNA | GeneChem (Shao et al., 2015). |
||
| Biological sample (human) | Ascending aorta | Zhongshan Hospital, Fudan University | Ascending aortic tissues from non-diseased and BAV-TAA patients. | |
| Antibody | Anti-DRP1 (D6C7) (Rabbit monoclonal) | Cell Signaling Technology | Cat # 8570S, RRID:AB_10950498 |
(1:1000), western blotting |
| Antibody | Anti-MFF (E5W4M) (Rabbit monoclonal) | Cell Signaling Technology | Cat # 84580, RRID:AB_2728769 |
(1:1000), western blotting |
| Antibody | Anti-Mitofusin-1 (D6E2S) (Rabbit monoclonal) | Cell Signaling Technology | Cat # 14739S, RRID:AB_2744531 |
(1:1000), western blotting |
| Antibody | Anti-Mitofusin-2 (D1E9) (Rabbit monoclonal) | Cell Signaling Technology | Cat # 11925, RRID:AB_2750893 |
(1:1000), western blotting |
| Antibody | Anti- Notch1 [EP1238Y] (Rabbit monoclonal) | Abcam | Cat # ab52627, RRID:AB_881725 |
(1:1000), western blotting |
| Antibody | Anti- TAGLN (SM22) (Rabbit polyclonal) | Abcam | Cat # ab14106, AB_443021 |
(1:1000), western blotting, (1:300) for IF |
| Antibody | Anti- Calponin (CNN1) (Rabbit monoclonal) | Abcam | Cat # ab46794, RRID:AB_2291941 |
(1:1000), western blotting, (1:300) for IF |
| Commercial assay or kit | Tetramethylrhodamine methyl ester (TMRM) | Thermo Fisher Scientific | Cat # I34361 | |
| Commercial assay or kit | MitoSOX | Thermo Fisher Scientific | Cat # M36008 | |
| Commercial assay or kit | MitoTracker | Thermo Fisher Scientific | Cat # M22426 | |
| Chemical compound, drug | Mdivi-1 | Sigma | Cat # M0199 | a final concentration of 30 μM. |
| Chemical compound, drug | Leflunomide | Sigma | Cat # L5025 | a final concentration of 75 μM. |
| Chemical compound, drug | Teriflunomide | Sigma | Cat # SML0936 | a final concentration of 75 μM. |
| Other | Commercial flexible PDMS membrane | Hangzhou Bald Advanced Materials | # KYQ-200 |