Fig. 4. (a) Schemes of the GlcNAc de novo hexosamine biosynthetic pathway (HBP) and salvage pathway from exogenous GlcNAc, showing a range of synthetic metabolic chemical reporters (MCRs). The engineered GlcNAc analogues enter the salvage pathway and are converted to the corresponding UDP-GlcNAc derivatives in the cell; subsequent OGT-catalysed O-GlcNAcylation enables labelling of the relevant O-GlcNAc modified proteins. (b) General workflow showing how the probes are applied for the identification of the O-GlcNAcylated proteins.95,104,105.
