FIG. 1.

Physical mapping of the DNA probes on S. aureus NCTC 8325 (29) restriction fragments. (A) PFGE macrorestriction patterns of S. aureus NCTC 8325 digested with SmaI, SgrAI, and AscI (lanes 1, 2, and 3, respectively). Restriction fragments are coded by descending molecular size. (B) Example of hybridization results with probe AW-15 to PFGE patterns of S. aureus NCTC 8325. Lanes are the same as for panel A. (C) Hybridization results of probe AW-15 depicted on the physical map of S. aureus NCTC 8325. (D) Mapping results of the 15 strain-specific DNA probes (AW-1 through AW-15) to the macro-restriction fragments of the S. aureus NCTC 8325 genome. NH, no hybridization of the strain-specific DNA probe to the macrorestriction fragments.