TABLE 2.
Summary of the demographic data and the sequence homologies from the RAPD-generated S. aureus DNA fragments with strain-specific characteristics (n = 15)
| Probe code | Origin | Source strain code | Mc resultb | Probe size (bp) | Specificityc | Nucleotide sequence homologyd
|
Protein sequence homologyd
|
||
|---|---|---|---|---|---|---|---|---|---|
| M13 | T7 | M13 | T7 | ||||||
| AW-1 | CDCa | SA-08 | R | 1,200 | 13 | NH | NH | NH | NH |
| AW-2 | CDC | SA-01 | R | 600 | 8 | NH | NH | NH | NH |
| AW-3 | CDC | SA-06 | I | 550 | 7 | NH | NH | HrmA | NH |
| AW-4 | CDC | SA-02 | R | 700 | 6 | S. aureus plasmid pSH6 | S. aureus plasmid pSH6 | S. aureus transposase IS257 | S. aureus transposase IS257 |
| AW-5 | Portugal | HPV107 | R | 400 | 3 | NH | NH | Lysostaphin precursor of S. simulans | NH |
| AW-6 | United States | BK1591 | R | 350 | 5 | NH | NH | NH | NH |
| AW-7 | Italy | 246D | R | 1,200 | 4 | NH | NH | NH | NH |
| AW-8 | Australia | WBG8217 | R | 350 | 5 | B. subtilis yqeV | B. subtilis yqeV | B. subtilis hypothetical protein YqeV | B. subtilis hypothetical protein YqeV |
| AW-9 | United States | BK1457 | R | 1,500 | 5 | NH | NH | NH | NH |
| AW-10 | United States | BK1461 | R | 400 | 6 | NH | NH | NH | NH |
| AW-11 | Australia | WBG8231 | R | 1,500 | 6 | NH | NH | NH | NH |
| AW-12 | CDC | SB-18 | R | 350 | 4 | NH | NH | NH | NH |
| AW-13 | Italy | 85CCH | R | 350 | 4 | NH | NH | NH | NH |
| AW-14 | United States | BK1563 | R | 650 | 4 | NH | NH | NH | NH |
| AW-15 | Italy | 76CCH | R | 300 | 5 | NH | NH | NH | NH |
a
CDC, Center for Disease Control and Prevention.
b
Mc; methicillin susceptibility disk-diffusion test; R, resistant; I, intermediate.
c
Number of strains hybridizing with a particular probe. The probes were tested on 14 CDC strains (Table 1, collection 7).
d
NH; no homology. Cloned DNA sequences of insert termini (M13 and T7) were analyzed for sequence homologies with nucleotide and protein sequences in the NCBI data bank by using the BLAST computer program (2).