TABLE 3.
Stability of the strain-differentiating DNA probes determined with S. aureus strains obtained from persistent nasal carriers
| Persistent carrier (sex)a | Strainb | Isolation date | Binary code (type)c | Result by indicated genotyping techniqued:
|
||||
|---|---|---|---|---|---|---|---|---|
| RAPD analysis | PFGE | spa polymorphism | Coagulase gene polymorphism | Presence/absence of cna | ||||
| 099 (F) | NC-711 | 1988 | 001010110101111 (5551) | CCC | J | 9 | F | − |
| NC-220 | September 1995 | 001010110101111 (5551) | CCC | J | 9 | F | − | |
| 060 (F) | NC-1740 | 1988 | 101010011100101 (21733) | FCC | M | 9 | D | − |
| NC-1733 | February 1995 | 101010011100101 (21733) | FCC | M | 5 | D | − | |
| 038 (F) | NC-1288 | 1988 | 000000011100111 (231) | EDE | G | 7 | B | − |
| NC-105 | June 1995 | 000000011100111 (231) | EDE | G | 7 | B | − | |
| 076 (F) | NC-705 | 1988 | 000010011100101 (1253) | AEA | A | 7 | A | + |
| NC-054 | May 1995 | 000010011100101 (1253) | AEA | A | 7 | A | + | |
| 145 (M) | NC-714 | 1988 | 000000010100101 (165) | BBB | B | 8 | B | + |
| NC-063 | May 1995 | 000000010100101 (165) | BBB | B | 8 | B | + | |
a
Persistency defined as 10 identical culture results from longitudinal sampling over 3 months (43).
b
NC, strain from nasal carrier. See Table 1, collection 8, for more details.
c
Overall results after hybridization with 15 strain-specific DNA probes (AW-1 through AW-15, respectively). The decimal number represents the binary type. See Materials and Methods for more information.
d
Results (except for presence/absence of cna) are given as codes for each technique. See Materials and Methods for more information.