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. 1999 Mar;37(3):664–674. doi: 10.1128/jcm.37.3.664-674.1999

TABLE 5.

Survey of geno- and phenotypic results for epidemic outbreak strains of MRSA and MSSA from Dutch hospitals and nursing homes

Cluster codea Epidemiological data
Isolation date (mo/yr) Binary codeb Binary typec Phage typed Ribotypee Result for indicated typing method:
Hospital Patient Isolate source PFGEf RAPD analysisg Coagulase gene polymorphismh cnai spaj mecAk mecIk mecR1k
5′ 3′
MSSA
 Ia A 1 Groin 11-96 101001111111101 21501 A A AAA −/− 11 − − + −
 Ib A 1 Sputum 11-96 101001111111101 21501 A A AAA A −/− 11 − − + −
 Ic A 1 Blood 11-96 101001111111101 21501 A A AAA A −/− 11 − − + −
 Id A 2 Sputum 11-96 101001111111101 21501 A A AAA A −/− 11 − − + −
 Ie A 3 Blood 07-96 101001111111101 21501 A A AAA A −/− 11 − − + −
 IIa B2 1 Pus 12-96 001000000100000 4128 A B BBB NR +/+ 5 − − + −
 IIb B1 1 Pus 10-96 001000000100000 4128 A B B1BB NR +/+ 5 − − + −
 IIc B1 2 Pus 08-96 001000000100000 4128 A B1 B1BB NR +/+ 10 − − + −
 IId B2 3 Pus 11-96 001000010100000 4256 A B2 B1BB NR +/+ 11 − − + −
 IIe B2 4 Pus 10-96 001000000100000 4128 A B BBB NR +/+ 5 − − + −
 IIIa B1 5 Pus 11-96 000000110101011 427 B C CCC NR −/− 10 − − + −
 IIIb B3 6 Urine 10-96 000000110101011 427 B C CCC NR −/− 10 − − + −
 IIIc B3 6 Urine 10-96 000000110101011 427 B C CCC NR −/− 10 − − + −
 IIId B3 (B1)l 7 Pus 12-95 010000110101011 8619 B C1 CCC NR −/− 10 − − + −
 IIIe B1 8 Urine 02-96 010000110101011 8619 B C1 CCC NR −/− 10 − − + −
 IVa C 1 Nose 11-96 001011010100010 5794 A D DDD B −/+ 7 − − + −
 IVb D (C)m 2 Exit site 04-96 001001010100010 4770 A D DDD B +/+ 9 − − + −
 IVc C 3 Nose 07-96 001011010100010 5794 A D1 DDD1 B −/+ 11 − − + −
 IVd C 4 Pus 08-96 001011010100010 5794 A D1 DDD1 B −/+ 11 − − + −
MRSA
 Va En 1 Unknown 01-96 110111111111111 28671 XVI-3 A E1 EEE1 C +/+ 10 + + + +
 Vb E 2 Unknown 01-96 110111111111111 28671 XVI-3 A E1 EEE1 C +/+ 10 + + + +
 Vc E 3 Unknown 01-96 110111111111111 28671 XVI-3 A E1 EEE1 C +/+ 10 + + + +
 Vd E 4 Unknown 01-96 110111111111111 28671 XVI-3 A E EEE1 C +/+ 10 + + + +
 Ve E 5 Unknown 01-96 110111111111111 28671 XVI-3 A E1 EEE1 C +/+ 10 + + + +
 VIa F1o 1 Unknown 10-96 001110010000000 7296 XVI-4 A F FFB NR +/+ 9 + − + −
 VIb F1 2 Unknown 05-96 001110010000000 7296 XVI-4 A F FFB NR +/+ 9 + − + −
 VIc F1 3 Nose 05-96 001110010000000 7296 XVI-4 A F FFB NR +/+ 9 + − + −
 VId F1 4 Unknown 07-96 001110010000000 7296 XVI-4 A F FFB NR +/+ 9 + − + −
 VIe F1 5 Unknown 04-96 001110010000000 7296 XVI-4 A F FFB NR +/+ 9 + − + −
 VIIa F2o 1 Nose 10-96 001110000000000 7168 XI-6 A F1 FFB NR +/+ 9 + − + −
 VIIb F2 2 Nose 10-96 001110000000000 7168 XI-6 A F1 FFB NR +/+ 9 + − + −
 VIIc F2 3 Nose 10-96 001110000000000 7168 XI-6 A F1 FFB NR +/+ 9 + − + −
 VIId F2 4 Nose 10-96 001110000000000 7168 XI-6 A F1 FFB NR +/+ 9 + − + −
 VIIe F2 5 Nose 10-96 001110000000000 7168 XI-6 A F1 FFB NR +/+ 9 + − + −
 VIIIa G 1 Sputum 01-96 100111110111101 20413 Z-72 A G GGF D −/− 10 + − + −
 VIIIb G 2 Unknown 03-96 100111110111101 20413 Z-72 A G GGF D −/− 10 + − + −
 VIIIc G 3 Unknown 04-96 100111110111101 20413 Z-72 A G GGF D −/− 10 + − + −
 VIIId G 4 Pus 04-96 100111110111101 20413 Z-72 A G GGF D −/− 10 + − + −
 VIIIe G 5 Nose 04-96 100111110111101 20413 Z-72 A G GGF D −/− 10 + − + −
 IXa H 1 Urine 01-96 101110010101101 23725 III-29 A H HAA D −/− 9 + − + −
 IXb H 1 Urine 01-96 101110010101101 23725 III-29 A H HAA D −/− 9 + − + −
 IXc H 2 Pus 01-96 101110010101101 23725 III-29 A H HAA D −/− 8 + − + −
 IXd H 3 Pus 01-96 101110010101101 23725 III-29 A H HAA D −/− 9 + − + −
 IXe H 4 Pus 01-96 101110010101101 23725 III-29 A H HAA D −/− 9 + − + −
 X1 I 1 Ven. line 01-93 001111110101011 8107 III-29 A I IAA D −/− 10 + − + −
 X2 I 2 Nose 01-93 001111110101011 8107 III-29 A I IAA D −/− 10 + − + −
 X3 I 3 Wound 02-93 001111110101011 8107 III-29 A I IAA D −/− 10 + − + −
 X4 I 4 Bile 02-93 001111110101011 8107 III-29 A I IAA D −/− 10 + − + −
 X5 I 5 Wound 02-93 001111110101011 8107 III-29 A I IAA D −/− 10 + − + −
a

See Table 1, collection 10 for more details. 

b

Overall results after hybridization with 15 strain-specific DNA probes (AW-1 through AW-15, respectively). 

c

Binary code transformed into a decimal number. 

d

MSSA strains have identical phage types within a cluster. Types were dropped on account of the extensive code. 

e

Results with EcoRI-digested genomic DNA. 

f

After SmaI macrorestriction analysis. 

g

The three letter code summarizes the typing results per primer used (first letter, primer ERIC-2; second letter, primer AP-1; third letter, primer AP-7). 

h

RFLP analysis of the coagulase gene and subsequent digestion of the amplicon with AluI. NR, no restriction sites on the amplicon. 

i

PCR result with cna gene-specific primers is presented before the shill, and the plus or minus after the shill describes the hybridization result with the PCR-generated, cna-specific DNA probe. 

j

Analysis of the so-called X region by PCR. Amplicons were digested with RsaI. The number of direct repeats in this region was determined after electrophoresis. 

k

−, absence of the mec-specific DNA regions analyzed by PCR; +, presence of the mec-specific DNA regions analyzed by PCR. 

l

Patient removed from hospital B3 to an annex, hospital B1. 

m

Patient was transferred from nursing home D to hospital C. 

n

Outbreak in a hospital on the Netherlands Antilles. 

o

F1 and F2 are subdivisions in different locations of the same hospital.