Fig. 2.

LINC00022 facilitates ESCC proliferation in vitro. (A) The knockdown efficiencies of three specific siRNAs on LINC00022 in KYSE150 and TE1 cells were tested by qRT-PCR, ***p < 0.001. (B) The expression of LINC00022 in KYSE70 cells infected with recombinant over-expressed lentivirus (OE-022) was determined by qRT-PCR, **p < 0.01. (C-E) CCK-8 assay was carried out to evaluate cell viability of KYSE150 and TE1 with LINC00022 knockdown (C, D), and KYSE70 with LINC00022 over-expression (E), *p < 0.05; **p < 0.01. (F-G) Ablation of LINC00022 attenuated the colony formation ability of KYSE150 and TE1 (F), while over-expression of LINC00022 enhanced the colony formation ability of KYSE70 (G), *p < 0.05; **p < 0.01; ***p < 0.001. (H-I) EdU staining assay was conducted to examine cell proliferation of KYSE150 and TE1 with LINC00022 knockdown (H), and KYSE70 with LINC00022 over-expression (I), *p < 0.05; **p < 0.01. Scale bar = 100 μm