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. 2021 Sep 20;40:294. doi: 10.1186/s13046-021-02096-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — FTO/LINC00022 axis drives ESCC tumorigenesis. (A) Deletion of LINC00022 attenuated the proliferative effects induced by FTO over-expression in KYSE70 cells, *p < 0.05. (B) Ectopic expression of FTO partially relieved the inhibitory effects of proliferation caused by LINC00022 ablation in KYSE150 cells, *p < 0.05; **p < 0.01. (C) The non-population-dependent growth ability of ESCC cells under the control of the FTO/LINC00022 axis was evaluated by the colony formation assay, *p < 0.05. (D-E) PI-labeling staining combined with flow cytometry indicated the role of FTO/LINC00022 axis in ESCC cell-cycle progression, *p < 0.05; **p < 0.01. (F-G) Western blot was used to investigate the changes of cell cycle regulator protein levels, p16, p21 and p53 in KYSE70 (F) and KYSE150 (G) cells following FTO-LINC00022 cross-talking. (H) Knockdown of LINC00022 fully rescued the growth promotion effect induced by ectopic FTO expression on KYSE70 cells in nude mice (n = 3) (left panel). The tumor volume was measured every three days from day 9 to day 21 after cell inoculation, and the curve was plotted (right panel), *p < 0.05. (I) Over-expression of FTO partially attenuated the inhibition of subcutaneous tumorigenicity caused by LINC00022 knockdown on KYSE150 cells in nude mice (n = 4) (left panel). The tumor volume was monitored every five days from day 10 to day 30 after cell inoculation, and the curve was generated (right panel), *p < 0.05