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Growth rate of PC-3 spheroids following treatment with RALA-AuNP and Radiation. 1000 PC-3 cells were seeded in a round bottom 96-well plate for 48 h, allowing tumorspheres to form. Tumorspheres were treated with citrate-AuNP or RALA-AuNP complexes at a w:w ratio of 25:1 µg for 6 h, then irradiated with 8 Gy using 160 kV X-rays. Tumoursphere growth rate and doubling time was measured daily using the Celli3Imager. Each treatment group corresponds to 40 spheroids
