Fig. 5.

Quantification of DNA double strand breaks following radiation and nanoparticle treatment. Cells seeded in chamber slides were treated with citrate-AuNP (1 µg) or RALA-AuNP (25 µg RALA: 1 µg AuNP) for 6 h. Excess AuNPs were removed, cells washed and then irradiated with 1 Gy. Cells were fixed and stained with anti-53BP1 and DAPI 30 min and 24 h post irradiation. Slides were imaged using a Lecia SP8 confocal microscope using × 40 objective. Z-stacks were taken at 0.5 µm steps with a minimum of 12 sections acquired. A Representative images of three independent replicates for PC-3 cells. Quantified 53BP1 foci indicating double strand break lesions in: B DU145, C PC-3 and D PNT2-C2 cells