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. 2021 Sep 20;21:504. doi: 10.1186/s12935-021-02180-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Knockdown of LINC01605 impairs the colony formation of CC cells. ShRNAs targeting LINC01605 (shRNA-1 and shRNA-2) were transfected into LoVo and Caco-2 cells. A LINC01605 expression in cells measured by RT-qPCR. B the number of colonies formed by LoVo and Caco-2 examined using colony formation assay. C the proliferative activity of LoVo and Caco-2 examined using CCK-8 assay. D the proportion of apoptotic cells in LoVo and Caco-2 cells measured using flow cytometry. E the number of apoptosis bodies in cells examined using TUNEL assay. F, G the altered migration and invasion ability of LoVo and Caco-2 cells measured using Transwell assay. H the number of tubes formed by HUVEC cells co-cultured with Caco-2 and LoVo cells evaluated using tube formation assay. Error bars represent means ± SD for three independent experiments (**p < 0.01, two-way ANOVA, followed by Tukey’s multiple comparison)