Fig. 4.

Knockdown of LINC01605 impairs the growth and metastatic activity of CC cells in vivo. LoVo and Caco-2 cells with stable low expression of LINC01605 were inoculated subcutaneously into NSG mice. A the tumor growth curves of each group of mice are summarized. B box plot for the average tumor weight. C representative images of xenograft tumors in nude mice after different treatments. D–G, immunohistochemical detection of KI67 (D), PCNA (E), VEGFA (F) and Caspase-3 (G) staining intensity in xenograft tumors formed by LoVo or Caco-2 cells. H, CC cells were injected intracardially into NSG mice, and the survival rate of NSG mice after cell inoculation was analyzed by Kaplan–Meier. I, J, 30 days after inoculation of CC cells, mice were euthanized, and the number of metastatic nodes was counted by extracting lung (I) and liver (J) tissues from mice. In A–G, I and J, each group contains eight mice; and in H, each group contains 10 mice, with each dot representing one mouse (**p < 0.01, one-way or two-way ANOVA, followed by Tukey’s multiple comparison)