Fig. 5.

Overexpression of SMYD2 or EP300 promotes malignant biological behavior in CC cells. A schematic diagram of LoVo and Caco-2 cell lines with SE-deleted constructed by Crispr-Cas9 system. B detection of LINC01605 expression in SE-deleted LoVo and Caco-2 cells by RT-qPCR. Overexpression plasmids of SMYD2 or EP300 were transfected into cells with stable low expression of LINC01605. C SMYD2 or EP300 expression in cells after co-transfection measured using Western blot. D LINC01605 expression in LoVo and Caco-2 cells by RT-qPCR. E the proliferative activity of LoVo and Caco-2 examined using CCK-8 assay. F the proportion of apoptotic cells in LoVo and Caco-2 cells measured using flow cytometry analysis. G the number of apoptosis bodies in cells determined using TUNEL assay. H, I, the altered migration and invasion ability of LoVo and Caco-2 cells measured using Transwell assay. J the number of tubes formed by HUVEC cells co-cultured with Caco-2 and LoVo cells examined using tube formation assay. Error bars represent means ± SD for three independent experiments (**p < 0.01, two-way ANOVA, followed by Tukey’s multiple comparison)