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. 2021 Sep 19;40:292. doi: 10.1186/s13046-021-02091-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — TAGLN and Src activation form a regulation loop. A Western blot analyzed TAGLN and p-Src (Try416) expression of cells treated with dasatinib (20 nM, 24 h). GAPDH was used as a loading control. B Western blot analyzed TAGLN and p-Src (Try416) expression of cells treated with Src inhibitor 1 (50 nM, 24 h). GAPDH was used as a loading control. C Lysates from cells transfected with vector or active Src Y416F plasmid (CA-Src416) were analyzed by SDS–PAGE and probed for TAGLN, p-Src (Try416) and GAPDH. D-E Representative images of IHC staining and scores of p-Src (Try416) (D)and TAGLN (E) from mice treated with DMSO or dasatinib (n = 8, *P < 0.05, **P < 0.01). Scale bar: 20 μm. F Western blot analyzed TAGLN and p-Src (Try416) expression of cells transfected with siControl or siTAGLN. GAPDH was used as a loading control. GWestern blot analyzed TAGLN and p-Src (Try416) expression of cells transfected with vector or TAGLN plasmid. GAPDH was used as a loading control. H Representative images (left) and scores (right) of p-Src (Try416) IHC staining of tumor tissues from mice bearing SK-OV-3 cells transfected with sh-control or sh-TAGLN (*P < 0.05). Scale bar: 20 μm. I Phase images showing typical morphology of SK-OV-3 and ES-2 cells transfected with siControl or siTAGLN. J Representative immunofluorescence images of SK-OV-3 and ES-2 cells transfected with siControl or siTAGLN for phalloidin (red) and with DAPI (blue). Scale bar: 20 μm. K Correlation between TAGLN and p-Src (Try416) protein expression in primary tumors of ovarian cancer tissues (n = 10 biological replicate samples). L Immunohistochemical analysis of TAGLN and p-Src (Try416) expression from primary tumors of EOC. The scores of p-Src (Try416) in the TAGLN-low group (n=16) and TAGLN-high group (n=17) was calculated