Table I.
Experimental Design to Determine the Effect of Factors on the Transfection Efficiency of Luc-MVs and Luc-EXOs
| Factors | Level 1 | Level 2 | Level 3 |
|---|---|---|---|
| Amount of Luc pDNA (μg/well)a | 0 | 0.5 | 1 |
| Total EV protein (μg)b | 6 | N/A | 12 |
| Type of EVs | EXOs | N/A | MVs |
| Incubation time (h)c | 24 | 48 | 72 |
a
Amount of Luc pDNA represents the amount of gWiz-Luc pDNA transfected into the donor cells using lipofectamine3000 in each well in a 24-well plate and the EVs were isolated from a single 24-well plate that was transfected with a total of either 0, 12, or 24μg pDNA.
b
Total EV protein represents the protein content in EXOs or MVs quantified by MicroBCA protein assay
c
Incubation time represents the time that recipient hCMEC/D3 cells were treated using Luc-EVs. N/A: not applicable