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. Author manuscript; available in PMC: 2021 Sep 20.
Published in final edited form as: Cell Rep. 2021 Sep 7;36(10):109674. doi: 10.1016/j.celrep.2021.109674

Figure 6. Testing a role of PGE2 signaling in promoting TSCs propagation and tumorigenesis using lineage tracing assay.

Figure 6.

(A) IF co-staining of TrCSC markers Krt15 (green) with CldU+ (red) slow-cycling cells.

(B) Predominant expression of Krt15 or Bmi1 in TSC C11 at 24 h after CRT.

(C) The procedure for marking Bmi1-Cre-derived single TSCs.

(D) IH tracing Bmi1-derived tumor clones (brown).

(E) Lineage tracing showing that Bmi1-CreER-derived clones include all four epithelial lineages in intestine.

(F) After TMX induction, association of CD68+ with Bmi1+ (green) single cells and Bmi1-derived tumor clones.

(G) Percentage of Bmi1+ single cells per small intestine with different therapies (*n = 2–4 mice).

(H) Quantification of Bmi1-Cre-derived tumor clones per small intestine with different therapies (*n = 2–3 mice).

*Because of the COVID-19 pandemic, the mouse experiment to increase the control group of animal number in the Bmi1-CreER:R26LSL-GFP:ApcMin/+-induced lineage assay was affected. T-tests, Means+/−SD.