Figure 3.

Time dependence of anti-inflammatory response of HEK cells. The inflammatory response was induced in HEK-TLR4 cell cultures by incubation with 100 ng/ml LPS and followed by exposure to 720 nm infrared illumination under the indicated conditions. Inflammatory response was determined by colorimetric assay (methods). The control condition represents the inflammatory response in the absence of Infrared exposure. Exposure to infrared was for 5, 10, and 15-min intervals, respectively. The intervals were repeated once every 12 h, over 48 h for a total of four Infrared exposure periods. The Inflammatory response was assayed by colorimetric assay at 12 h after the final Infrared treatment. Data represent the mean ± SE of three independent experiments (N = 3). The asterisks indicate significance level of the differences: ****p-value < 0.0001