Fig. 3.
Representative ddPCR results for probe 5 (DDX41) are shown for DNA extracted from the blood pellet (a), tumor tissue (b), and CSF supernatant (c). Concentrations of the ddPCR reaction are given in ng/uL for mutant and wild-type. Only wild-type (green, HEX) signal is detected in DNA extracted from blood (a). Both wild-type (green, HEX) and mutant (orange, FAM) signals were detected in DNA extracted from tumor tissue (b). cfDNA extracted from CSF contained wild-type signal and a small amount of mutant signal which was unreliably detected across experimental duplicates (c)