Skip to main content
. 2021 Jun 7;17(1):144–151. doi: 10.4103/1673-5374.314114

Figure 3.

Figure 3

circPrkcsh expression influences the TNF-α-induced expression of inflammatory-related cytokines in astrocytes.

(A, B) Expression changes of circPrkcsh over time or with the concentration gradient of TNF-α in treated astrocytes were detected using RT-qPCR. (C, D) circPrkcsh expression in astrocytes with siRNA or circPrkcsh-overexpression plasmid transfection, as well as with the negative control (NC). (E–I) Expression of Ccl2, IL-1β, CD86, IL-10, and CD206 in astrocytes transfected with siRNA or plasmid, as detected by RT-qPCR. (J–L) Secretion of CCL2, IL-1β, and IL-10 from astrocytes treated with siRNA or plasmid, as measured using ELISA. In E–L, the first NC group is compared with the siRNA group, and was treated with siRNA-NC. The second NC group is compared with the OE group, with plasmid transfection, and was treated with plasmid-NC. Data (expressed as the fold change compared with the NC group) are expressed as the mean ± SD based on triplicate independent experiments (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Student’s t-test). Ccl2: Chemokine CC motif ligand 2; ELISA: enzyme-linked immunosorbent assay; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL: interleukin; OE: overexpression; RT-qPCR: reverse transcription-quantitative polymerase chain reaction; siRNA: small interfering RNA; TNF-α: tumor necrosis factor-α.