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Experimental protocol.
Spinal cord injury (SCI) was induced at T9-10 vertebral levels. Alda-1 (a selective activator of aldehyde dehydrogenase 2) was administered to animals initially at 2 hours post-SCI and the same dose was repeated once daily until the endpoint (30 days). Studies on Basso Mouse Scale (BMS) scores were performed 3 days before SCI, followed by day 3 after SCI and days indicated. Age, sex and environment-matched control (sham) mice were be used in all studies.
