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. 2021 Jun 7;17(1):194–202. doi: 10.4103/1673-5374.314323

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Copyright: © Neural Regeneration Research

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Characterization and tracing of BMSC-EXOs.

(A–D) Morphology (A, floating method; B, dripping method), nanoparticle size distribution (C), and marker detection for BMSC-EXOs. Exosomes prepared by the floating method were more stereoscopic and clearer in morphology than those prepared by the dripping method. (D–F) Western blot, transmission electron microscope, and nanoparticle tracking analysis of BMSC-EXOs. (E) BMSC-EXOs labeled for PKH26 were observed using immunofluorescence in pericytes cultured for 6 hours in vitro. (F) BMSC-EXOs were found in spinal cord lesions 3 days after SCI. Scale bars: 20 µm in E, 100 nm in A, B; 200 µm in F. BMSC-EXOs: Bone mesenchymal stem cell-derived exosomes; EXO: exosome; PDGFR: platelet-derived growth factor receptor.