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. 2021 Jun 7;17(1):194–202. doi: 10.4103/1673-5374.314323

Figure 5.

Figure 5

Compound stimulus induces pyroptosis in pericytes in vitro.

(A) Double staining for propidium iodide and Hoechst 33342 in pericytes. Scale bars: 100 µm. (B, E) Expression of pro-caspase 1 (fold change to sham group) was assessed by western blot. (C) The dead cells in cultured pericytes were detected using Hoechst 33342/PI. (D) Cell viability was assessed by the MTT assay. Data are presented as mean ± SD. The experiment was repeated six, six and three times in C, D and E, respectively. †P < 0.05, vs. ATP group; ‡P < 0.05, vs. IFN-γ + TNF-α + LPS + Lipo group (Student’s t-test). IFN-γ: Interferon-γ; Lipo: Lipofectamine 3000; LPS: lipopolysaccharide; MTT: methylthiazolyldiphenyl-tetrazolium bromide; TNF-α: tumor necrosis factor α.