Fig. 2. Effect of 2′-FL supplementation on gut barrier integrity. FD4 flux (paracellular pathway; A), HRP flux (transcellular pathway; B) in the cecum and IL-22 gene expression in the ileum (C) of mice fed an LF or HF with or without 10% 2′-FL supplementation diet for 8 weeks. Two-way analysis of variance followed by Tukey's or Sidak's post-hoc test was used to determine differences among groups. Values are means ± SEMs; n = 8/group. Asterisk (*) or sharp (^#) denotes significant differences between LF vs. HF or Con vs. 2′-FL respectively at P < 0.05. NS, not significant. FD4, fluorescein isothiocyanate–dextran 4000; HF, high fat; HF/CON, HF without 2′-FL; HF/2′-FL, HF with 2′-FL (w/w) in diet; HRP, horseradish peroxidase; LF, low fat; LF/CON, LF without 2′-FL; LF/2′-FL, LF with 2′-FL (w/w) in diet; 2′-FL, 2-fucosyllactose.