Fig. 4. Effect of 2′-FL supplementation on cognitive functions was determined by Y-maze (A and B), open-field (C and D) and novel object recognition (E) tests in mice fed an LF or HF with or without 10% 2′-FL supplementation diet for 8 weeks. Two-way analysis of variance followed by Tukey's or Sidak's post-hoc test was used to determine differences among groups. Values are means ± SEMs; n = 8/group. Asterisk (*) denotes significant differences between LF vs. HF at P < 0.05. NS, not significant. HF, high fat; HF/CON, HF without 2′-FL; HF/2′-FL, HF with 2′-FL (w/w) in diet; LF, low fat; LF/CON, LF without 2′-FL; LF/2′-FL, LF with 2′-FL (w/w) in diet; 2′-FL, fucosyllactose.