Figure 1.

SM04755 was a potent inhibitor of canonical Wnt signaling and induced tenocyte differentiation in rTDSCs. (A) Doseresponse of TCF/LEF promoter‐driven or EF1α promoter‐driven luciferase reporters in SW480 cells treated with SM04755 or DMSO for 48 h (n = 4, mean ± SD). (B) Expression of Wnt pathway genes in rTDSCs measured by qRT‐PCR. Fold change relative to DMSO (n = 3, mean ± SEM). (C) Representative images of immunostaining for tenocyte markers of rTDSCs treated with SM04755 (1.5 μM) or DMSO for 4 days. (D) Dose‐response quantification of the percent of total cells that were positive for the tenocyte markers in (C) (n = 4, mean ± SD). BMP + FGF was used as a positive control. Scale bars: 200 μm; **p < .01, ***p < .001, t‐test