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. 2020 Dec 30;99(8):807–813. doi: 10.1002/cyto.a.24297

FIGURE 3.

FIGURE 3

AMH production and number of FSHR+AMH+ GCs at 4 weeks after Cy exposure. (A) Schematic of the in vivo study. Cy (100 mg/kg) was intraperitoneally injected into C57BL/6 mice 6 times over 2 weeks. GCs were isolated from PMSG‐stimulated ovaries at 4 weeks after Cy exposure. (B) Body growth was measured for 6 weeks. (C) Ovary weight was measured. (D) Serum AMH levels were measured by ELISA. (E) The number of FSHR+AMH+ GCs per ovary were counted using counting beads. (F,G) The mean fluorescence intensity (MFI) of FSHR and AMH in FSHR+AMH+ GCs were calculated using the isotype control antibody‐stained cells (gray line). Untreated: untreated control mice; Cy: cyclophosphamide‐treated mice. Data represent the mean ± s.e.m. (n = 8/group). n.s., not significant. Ab, antibody; AMH, anti‐müllerian hormone; Cy, cyclophosphamide; FSHR, follicle‐stimulating hormone receptor; GC, granulosa cells; PMSG, pregnant mare's serum gonadotropin [Color figure can be viewed at wileyonlinelibrary.com]