FIGURE 1.

Effects of IS and Klotho on HUVECs viability. Cell viability was detected by CCK‐8 assay. (A) Cells were exposed to 50 μg/mL of IS for 12, 24, and 48 h. (B) Cells were exposed to various concentrations of IS (5, 25, and 50 μg/mL) for 48 h. (C) Cells were pretreated with Klotho (1, 10, and 100 μg/L) for 1 h, then exposed to IS (50 μg/mL) for 48 h. Data were shown as mean ± SD. Statistical differences were expressed as *P < 0.01 versus control; #P < 0.01 versus IS alone. HUVECs, human vein umbilical endothelial cells; IS, indoxyl sulfate