FIGURE 4.

Effects of IS and Klotho on apoptosis in HUVECs. Cells were pretreated with Klotho (100 μg/L) for 1 h, then incubated with IS (50 μg/mL) for further 48 h with or without Compound C (10 μmol/L). (A) Cells apoptosis were determined by flow cytometry. (B) Western blotting analysis for caspase‐3. (C) Hoechst staining (×200). Arrow indicated apoptosis‐induced chromatin condensation. Data were shown as mean ±SD. Statistical differences were expressed as * P < 0.01 versus IS alone; #P < 0.01 versus IS and Klotho treatment together; ##P < 0.05 versus IS and Klotho treatment together. HUVECs, human vein umbilical endothelial cells; IS, indoxyl sulfate