FIGURE 5.

Effects of IS and Klotho on the expression of ICAM‐1 and VCAM‐1 in HUVECs. Cells were pretreated with Klotho (100 μg/L) for 1 h, then incubated with IS (50 μg/mL) for further 48 h with or without Compound C (10 μmol/L). (A) Western blotting analysis for ICAM‐1. (B) Western blotting analysis for VCAM‐1. Data were shown as mean ±SD. Statistical differences were expressed as * P < 0.01 versus IS treatment alone; ** P < 0.05 versus IS alone; #P < 0.05 versus IS and Klotho treatment together. HUVECs, human vein umbilical endothelial cells; IS, indoxyl sulfate; ICAM‐1, intercellular adhesion molecule‐1; VCAM‐1, vascular cell adhesion molecule‐1