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. 2020 Jun 15;158(3):724–736. doi: 10.1111/jnc.15096

FIGURE 5.

FIGURE 5

Increased CatB and translocation of p65 in cerebral endothelial cells of mice after systemic P. gingivalis infection. (a) Time schedule of systemic P. gingivalis infection and behavior test. (b) The immunofluorescent CLMS images of brain slice stained with CatB (green), CD31 (red), and Hoechst (blue) after systemic P. gingivalis infection. (c) The immunofluorescent CLMS images of brain slice stained with p65 (green), CD31 (red), and Hoechst (blue) after systemic P. gingivalis infection. Dash lines marked the location of blood vessels. (d) The quantitative analysis of CatB fluorescence density in CD31+ vessels in the images in (b). (e) Co‐localization analysis of p65 and Hoechst in the images in (c) using ImageJ (COLOC 2 plugin of Fiji is just ImageJ). Three mice per group were used and each mouse has its own color. Asterisks indicate a statistically significant difference versus “Saline” group using multiple t‐test. *p < .05, **p < .01. Scale bars in b: 50 μm; c: 10 μm