A, Plasma cGMP levels from blood taken immediately prior to sacrifice (n=10-17). B, Left ventricular (LV) tissue levels of cGMP (n=5-9). C, Protein expression for phosphorylated myosin binding protein-C at Ser273, normalized to GAPDH (n=5-7) and D, Protein expression for calcineurin from cardiac tissue, normalized to GAPDH (n=5-7). Results are expressed as mean ± SEM. Comparisons were performed using 1-way ANOVA with Tukey’s post-test (A-B) or Mann-Whitney U test (C-D).