Figure 9. Postnatal En1^Cre/+;Atoh1^fl/- mice have abnormal motor coordination, enhanced tremor and altered vocalization behavior.

(A) Timed-series photos of Atoh1^fl/+ (gray) and En1^Cre/+;Atoh1^fl/- (orange) mice. En1^Cre/+;Atoh1^fl/- mice have a wide stance (blue arrows) and fall on their backs (red arrows). (B) Time to right in the righting reflex of P6, P8, and P10 mice (top) and percentage of failed trials (bottom). (C) Representative ultrasonic vocalization traces with intensity of the black line representing the power of the vocalization frequency (filled circles in D and E). (D) Duration of vocalizations. (E) Frequency of vocalizations. (F) Representative tracks of mice in the open field (filled circles in G and H). Box measures 40x40 cm. (G) Movement speed. (H) Number of movements. (I) Representative power spectra of tremor recordings. (J) Tremor power at different frequencies. (K) Peak tremor power. N-numbers: Atoh1^fl/+ (gray): N=10–15 mice; En1^Cre/+;Atoh1^fl/+ (green): N=9–15 mice; Atoh1^fl/- (pink): N=11–15 mice; En1^Cre/+;Atoh1^fl/- (orange): N=11–14 mice. Significance was determined using a non-parametric Kruskal-Wallis test followed by a Tukey-Kramer post-hoc test. *p<0.05. The raw data and specific p-values for all the comparisons are presented in Figure 9—source data 1.