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. 2021 Sep 11;2021:1496101. doi: 10.1155/2021/1496101

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Copyright © 2021 Yongbin Ma et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of hUCMSCs and hUCMSC-EVs. (a) Morphology of passage 3 hUCMSCs under white light microscope, magnification: ×40. (b, c) Induction of osteogenic (alkaline phosphatase stained) and adipogenic differentiation (Oil Red O stained) of hUCMSCs, magnification: ×200. (d) Phenotypic markers related to hUCMSCs by flow cytometry analysis. (e) Representative images of hUCMSC-EVs using a transmission electron microscope (TEM), scale bar: 100 nm. (f) The particle size distribution and concentration of hUCMSC-EVs by nanoparticle tracking analysis (NTA). (g) Western blot analysis of CD63 expression in hUCMSC-EVs and hUCMSCs. Supernatant obtained during EV isolation by ultracentrifugation was regarded as negative control.