Figure 2.

Enhancement of RGC axonal outgrowth in the presence of appropriate post-synaptic target tissue

(A–C) RGC cellular aggregates (top) were grown (A) by themselves or in the presence of explants of either (B) LGN or (C) olfactory bulb tissue (bottom).

(D) Average RGC axon length was significantly increased in the presence of LGN explants.

(E and F) Sholl analyses indicated that significantly more RGC neurites grew in closer proximity to explants compared with olfactory bulb controls. (E) Distance from RGC, (F) distance from explant.

(G and H) Representative tracings demonstrating RGC neurite outgrowth after co-culture with (G) LGN or (H) olfactory bulb. After 1 week in culture the distance of the longest neurite within 10° steps from the explant was measured.

(I and J) Co-culture with (I) LGN explants specifically increased RGC outgrowth compared with (J) controls.

(K) RGC axons that grew closer to LGN explants were significantly longer.

Error bars represent SEM, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005, ^∗∗∗∗p < 0.001. n = 7 separate differentiation experiments each using the H7 line of hESCs and the H3 line of hiPSCs Scale bar, 500 μm.