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. 2021 Jun 10;16(9):2228–2241. doi: 10.1016/j.stemcr.2021.05.009

Figure 3.

Figure 3

Generation of assembloids from retinal and brain organoids

(A–D) After a total of 50 days of differentiation, retinal organoids and brain organoids were fused together to form assembloids. (A) Retinal, (B) brain, (C) assembloid. (D) Magnified region of box shown in panel C to demonstrate extension of tdTomato-expressing RGC axons into brain organoid.

(E–G) After 1 week, multiple RGC axons extended into CTIP2-positive brain organoids (E and F) and bundled together to form large axonal tracts (G). Box in panel E is magnified in panel F.

(H and I) The active growth of RGC axons was apparent by the presence of growth cone-like structures at the leading edge of RGC axons within brain organoids. Box in panel H is magnified in panel I. n = 6 separate differentiation experiments each using the H7 and H9 lines of hESCs.

Scale bars: (A–C) 500 μm, (D) 50 μm, (E) 300 μm, (F) 50 μm, (G) 200 μm, (H) 50 μm, (I) 30 μm.