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. 2021 May 20;16(9):2169–2181. doi: 10.1016/j.stemcr.2021.04.018

Figure 1.

Figure 1

Generation of DMD hiPSC-CMs

(A) hiPSC differentiation protocol for the generation of hiPSC-CMs. CHIR, CHIR-99021; IWR, IWR-1; ins, insulin; gluc, glucose; lac, lactate. hiPSCs were differentiated in medium supplemented with B27 minus insulin (ins). On day 6, the medium was supplemented with B27, including insulin. On day 10, hiPSC-CMs were cultured in medium without glucose supplemented with B27 and lactate.

(B) Representative micrograph in which hiPSCs were stained with pluripotent stem cell markers OCT4, NANOG, and SOX2.

(C) Representative micrograph in which hiPSC-CMs were stained with cardiac troponin T, α-actinin, and DAPI.

(D) Representative micrograph in which healthy and DMD hiPSC-CMs were stained with dystrophin, cardiac troponin T, and DAPI.

(E) Endogenous dystrophin (DMD) and utrophin (UTR) expression levels were determined by qRT-PCR in hiPSC-CMs (n = 6 independent experiments). Data shown as the mean ± SEM. Student's t test was used to calculate significance.