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. 2021 May 20;16(9):2169–2181. doi: 10.1016/j.stemcr.2021.04.018

Figure 4.

Figure 4

DMD hiPSC-CMs exhibit telomere shortening and DNA damage response

(A) Telomere length (TelC) was quantified by immunofluorescence staining (Q-FISH) relative to nuclear DAPI staining for hiPSC and hiPSC-CMs.

(B) Representative micrographs of hiPSC-CMs stained with cardiac troponin T, TelC and DAPI are shown.

(C) Quantification of hiPSC telomere using Q-FISH (n = 3 independent experiments, 17–86 cells analyzed).

(D) Telomere Q-FISH reveals progressive telomere loss in DMD hiPSC-CMs between days 20 and 30 (n = 3 independent experiments, 11–179 cells analyzed).

(E) hiPSC-CMs were devoid of EdU between days 20 and 30. Data are shown as violin plots where blue median and gray quartiles are shown. Student's t test was used to calculate significance.