Figure 3.

Sustained mTORC1 activation from loss of SESTRIN1,2 in aging induces loss of MuSCs

(A) Representative images of H&E staining in TA muscle sections from middle-aged mice (14 months). Scale bars, 100 μm.

(B) Size distributions of WT and SKO myofibers in TA muscle sections from middle-aged SKO and WT mice (WT, n = 5 mice; SKO, n = 3 mice). Inset shows mean size per condition. Statistical comparisons are two-sided, unpaired Student’s t tests with Holm multiple testing correction for the histogram.

(C) Quantification of TUNEL⁺ (plain) and TUNEL⁻ (stippled) PAX7⁺ cells as a fraction of total nuclei in TA muscle sections from 14-month-old SKO and WT mice (WT, n = 4 mice; SKO, n = 4 mice). Statistical comparisons are two-sided Mann-Whitney U tests. All data are shown as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001. See also Figure S4.