Figure 2.

Expression of stem cell, neuronal progenitor cell, neuronal, and glial cell markers in iNeurons co-cultured with astrocytes

(A) RNA-seq was performed on several representative MEA wells containing iNeurons co-cultured with rat astrocytes.

(B) Heatmap showing gene expression levels of stem cell/NPC genes (top section), neuronal and synaptic genes (middle section), and glial genes (bottom section), including rat homologs (left section, astrocyte samples), and human homologs (right section, iNeuron samples) in LH1 (n = 3), LH2 (n = 3), HH1 (n = 2), and HH2 (n = 2) iNeurons co-cultured with rat astrocytes. For iNeuron gene expression profiles, reads uniquely mapping to the human genome were extracted. For astrocyte gene expression profiles, reads uniquely mapping to the rat genome were extracted. Voom-transformed and batch-corrected counts per million (log2 scale) are shown.