Figure 1.

Identification of P5C3, a human mAb with high affinity binding to trimeric spike protein with mutations found in VOCs

(A) Binding of P5C3 and three benchmark anti-SARS-CoV-2 therapeutic mAbs to 10 trimeric Spike proteins produced with mutations found in VOCs. Representative of two to four independent experiments with each concentration response tested in duplicate. Mean values ± SEM are shown.

(B) Competitive binding studies between antibodies binding to the Spike RBD protein. RBC-coupled beads pre-incubated with saturating concentrations of competitor antibody were used for binding studies with mAbs or ACE2. Competitors induced either strong blocking (red boxes), partial competition (orange boxes), or non-competitive (white boxes) binding with the corresponding mAb to RBD.