Figure 2.

Chd4 controls the expression of cell-cycle regulators and lineage-specific genes in SCs

(A) Volcano plot (−log₁₀(p) versus log₂(fold change)) showing RNA-seq results from Chd4^fl/fl SCs transduced with Ad-CRE or Ad-GFP (control) (n = 4 independent experiments).

(B) GO analysis of the Biological Process database and Hallmark database of the significantly upregulated and downregulated genes.

(C) Volcano plot showing RNA-seq results from FACS-purified SCs at 3 days after TMX-induced muscle injury, in Chd4^fl/fl and Chd4^ΔPax7ER mice (n = 4 mice/group).

(D) TSEA analyses comparing gene expression of Chd4^fl/fl SCs transduced with Ad-CRE or Ad-GFP.

(E) TSEA analyses of gene expression from SCs purified from TMX-treated Chd4^fl/fl or Chd4^ΔPax7ER mice at 3 days after muscle injury.

(F) Relative mRNA expression of the indicated genes in Chd4^fl/f or Chd4^ΔPax7ER mice SCs at 3 days after injury (n = 4 mice/group). Data were normalized to housekeeping gene Rpl7, with the Chd4^fl/f set to 1.

(G and H) Functional annotation clustering based on DAVID biological processes of upregulated genes (G) or downregulated genes (H) in FACS-purified SCs, as in (C).