Figure 1.

DNA methylation suppresses XIST expression after erosion of dosage compensation

(A) Bisulfite sequence analysis at XIST in hPSCs and a somatic cell line. The percentage of XIST expression state was determined by RNA-FISH experiments.

(B) Experimental scheme for motor neuron differentiation with 5azaC.

(C) Immunofluorescence staining (ISL1) combined with XIST RNA-FISH at day 14. The scale bar represents 10 μm.

(D and E) Quantification of ISL1- (D) and XIST-positive (E) cells relative to DAPI in iPS18a and HUES21 p34. Each dot indicates the percentage of ISL- or XIST-positive cells in the observed area in two independent experiments. In each group, >424 cells were analyzed.

(F and G) Immunofluorescence staining (F) and quantification (G) of cells with XIST mono- and biallelic expression. The arrowheads indicate cells with biallelic XIST expression. The scale bar represents 10 μm. n means the number of cells analyzed.