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. 2021 Apr 22;16(9):2213–2227. doi: 10.1016/j.stemcr.2021.03.029

Figure 2.

Figure 2

Co-culturing of MNs and myotubes in microfluidic devices leads to NMJ formation

(A) Bright-field micrographs of MN and myotube (Myo) compartments in the XC150 device on day 28. Insets: magnification of axonal migration through microgrooves (arrowheads). Scale bars, 100 μm and 50 μm (insets).

(B and C) Confocal micrograph of NMJ formation are shown as co-localization of presynaptic marker synaptophysin (SYP) and acetylcholine receptor marker α-bungarotoxin (Btx) on MyHC-labeled myotubes. Insets: magnification of co-localizations (arrowheads). Scale bars, 25 μm and 10 μm (insets).