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. 2021 Apr 22;16(9):2213–2227. doi: 10.1016/j.stemcr.2021.03.029

Figure 3.

Figure 3

Agrin and laminin improve NMJ formation in microfluidic devices

(A and B) Confocal micrographs of NMJs in agrin (0.01 μg/mL)- and laminin (20 μg/mL)-supplemented (A/L) and untreated (Con) conditions on day 28 in XC150 devices. Single contact point NMJs (A). Multiple contact point NMJs (B). Scale bars, 10 μm. Arrowheads: Btx-SYP/NEFH co-localizations.

(C) Number of Btx and SYP/NEFH co-localizations per myotube.

(D) Quantifications of NMJ single and multiple contact point morphology.

(E and F) Representative scanning electron microscopy (SEM) images of NMJ formation in SND75 devices on day 28 in A/L-supplemented and control conditions. Single contact point NMJs (E). Multiple contact point NMJs (F). Insets: magnifications of NMJ (arrowheads). Scale bars, 2 μm and 1 μm (insets). (C) Unpaired t test. (D) One-way ANOVA with Tukey's multiple comparisons test. Mean ± SEM of four biological replicates. ∗p < 0.05, ∗∗p < 0.01.

See also Figures S2 and S3.