Figure 5.

NMJs are impaired in ALS

(A) Confocal micrographs of NMJs with A/L from ALS-FUS (P525L, R521H) and isogenic control (P525P, R521R) MN/myotube co-cultures on day 28. Scale bars, 10 μm. Arrowheads: Btx-SYP/NEFH co-localizations.

(B) Quantification of NMJs as Btx and SYP/NEFH co-localizations and expressed per myotube.

(C) Quantification of NMJ single and multiple contact point morphology. Mean ± SEM of three to four biological replicates. (B) Unpaired t test or Mann-Whitney test. (C) Kruskal-Wallis test with Dunn's multiple comparisons test. ^∗p < 0.05, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.

See also Figures S4, S5, and S7.